3.3 EFFECT OF A NOVEL COMPOUND ON FUNGAL CONTAMINATION.
Estelle Levetin, The University of Tulsa, Tulsa, OK
Fungal contamination of building materials, especially sheetrock
and ceiling tiles, is common following moisture damage. Compounds
that can prevent fungal growth would be useful pretreatments during
for the manufacture of these materials. The Aerobiology Lab has recently
tested a novel compound for this purpose. The compound is described
as catalyst altered water and has a pH of approximately 12.63. Cellulose-based
acoustic ceiling tiles and gypsum-based sheetrock were cut into 36
cm2 squares. Ceiling tile squares (CTS) and sheetrock squares (SRS)
were sterilized by autoclaving for 25 min at 121 C. CTS and SRS were
saturated with sterile distilled water or various concentrations of
the test compound. Saturated materials were inoculated with 10 l of
a spore suspension of fungi commonly isolated from buildings including
Stachybotrys chartarum, Cladosporium cladosporoides, Aspergillus
versicolor, Alternaria alternata, and Penicllium sp.
Fungal growth was measured weekly for 4 weeks. In addition, spore
germination experiments were conducted in 96-well microtiter plates.
Results showed that the test compound was effective in preventing
fungal growth on ceiling tiles and 75% solutions inhibited spore germination.
CTS were also soaked in the test compound, dried, and stored for varying
periods of time to simulate the use during manufacturing. The treated
tiles were later saturated with distilled water and inoculated with
spore suspensions. Fungal growth was inhibited on the treated tiles.
By contrast, the test compound was not effective on sheetrock. This
was possibly due to the buffering capacity of the gypsum. Results
suggest this compound may have some effectiveness in the ceiling tile
Last updated: March 3, 2011